[HTML][HTML] Rapid ex vivo expansion of human umbilical cord hematopoietic progenitors using a novel culture system
H Kawada, K Ando, T Tsuji, Y Shimakura… - Experimental …, 1999 - Elsevier
Cell numbers limit the widespread clinical use of cord blood (CB) for gene therapy and
marrow replacement in adults; a simple and effective method for ex vivo expansion of CB
primitive progenitor cells (PPC) is required. Recently, the combination of thrombopoietin
(TPO) and Flk-2/Flt-3 ligand (FL-2) was reported to support slow proliferation of CB-PPC in
stroma-free liquid culture. We established a novel culture system in which the murine
stromal cell line HESS-5 dramatically supports the rapid expansion of cryopreserved CB …
marrow replacement in adults; a simple and effective method for ex vivo expansion of CB
primitive progenitor cells (PPC) is required. Recently, the combination of thrombopoietin
(TPO) and Flk-2/Flt-3 ligand (FL-2) was reported to support slow proliferation of CB-PPC in
stroma-free liquid culture. We established a novel culture system in which the murine
stromal cell line HESS-5 dramatically supports the rapid expansion of cryopreserved CB …
