BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity
N Saeki, H Tokuo, M Ikebe - Journal of Biological Chemistry, 2005 - ASBMB
Myosin IXb, a member of the myosin superfamily, is a molecular motor that possesses a
GTPase activating protein (GAP) for Rho. Through the yeast two-hybrid screening using the
tail domain of myosin IXb as bait we found BIG1, a guanine nucleotide exchange factor for
ADP-ribosylation factor (Arf1), as a potential binding partner for myosin IXb. The interaction
between myosin IXb and BIG1 was demonstrated by co-immunoprecipitation of endogenous
myosin IXb and BIG1 with anti-BIG1 antibodies in normal rat kidney cells. Using the isolated …
GTPase activating protein (GAP) for Rho. Through the yeast two-hybrid screening using the
tail domain of myosin IXb as bait we found BIG1, a guanine nucleotide exchange factor for
ADP-ribosylation factor (Arf1), as a potential binding partner for myosin IXb. The interaction
between myosin IXb and BIG1 was demonstrated by co-immunoprecipitation of endogenous
myosin IXb and BIG1 with anti-BIG1 antibodies in normal rat kidney cells. Using the isolated …
