An efficient method to isolate and culture mouse Kupffer cells
P Li, J Li, M Li, J Gong, K He - Immunology letters, 2014 - Elsevier
Kupffer cells (KCs) play an essential role in the physiological and pathological functions of
the liver. Although the isolation methods of KCs have been well-described, most of them are
sophisticated and time-consuming. In addition, these methods are mainly used for isolating
the KCs of the human and rat. In this study, a three-step procedure was applied to isolate
KCs in sufficient number and purity from mouse liver, including the techniques of enzymatic
tissue treatment, gradient centrifugation, and selective adherence. F4/80 …
the liver. Although the isolation methods of KCs have been well-described, most of them are
sophisticated and time-consuming. In addition, these methods are mainly used for isolating
the KCs of the human and rat. In this study, a three-step procedure was applied to isolate
KCs in sufficient number and purity from mouse liver, including the techniques of enzymatic
tissue treatment, gradient centrifugation, and selective adherence. F4/80 …
