Simultaneous Detection of Ca2+ and Diacylglycerol Signaling in Living Cells
2012•journals.plos.org
Phospholipase C produces two second messengers-diacylglycerol (DAG), which remains in
the membrane, and inositol triphosphate (IP3), which triggers the release of calcium ions
(Ca2+) from intracellular stores. Genetically encoded sensors based on a single circularly
permuted fluorescent protein (FP) are robust tools for studying intracellular Ca2+ dynamics.
We have developed a robust sensor for DAG based on a circularly permuted green FP that
can be co-imaged with the red fluorescent Ca2+ sensor R-GECO for simultaneous …
the membrane, and inositol triphosphate (IP3), which triggers the release of calcium ions
(Ca2+) from intracellular stores. Genetically encoded sensors based on a single circularly
permuted fluorescent protein (FP) are robust tools for studying intracellular Ca2+ dynamics.
We have developed a robust sensor for DAG based on a circularly permuted green FP that
can be co-imaged with the red fluorescent Ca2+ sensor R-GECO for simultaneous …
Phospholipase C produces two second messengers - diacylglycerol (DAG), which remains in the membrane, and inositol triphosphate (IP3), which triggers the release of calcium ions (Ca2+) from intracellular stores. Genetically encoded sensors based on a single circularly permuted fluorescent protein (FP) are robust tools for studying intracellular Ca2+ dynamics. We have developed a robust sensor for DAG based on a circularly permuted green FP that can be co-imaged with the red fluorescent Ca2+ sensor R-GECO for simultaneous measurement of both second messengers.
PLOS