Insulin secretion induced by glucose-dependent insulinotropic polypeptide requires phosphatidylinositol 3-kinase γ in rodent and human β-cells
J Kolic, AF Spigelman, AM Smith, JEM Fox… - Journal of Biological …, 2014 - ASBMB
PI3Kγ, a G-protein-coupled type 1B phosphoinositol 3-kinase, exhibits a basal glucose-
independent activity in β-cells and can be activated by the glucose-dependent insulinotropic
polypeptide (GIP). We therefore investigated the role of the PI3Kγ catalytic subunit (p110γ) in
insulin secretion and β-cell exocytosis stimulated by GIP. We inhibited p110γ with
AS604850 (1 µmol/liter) or knocked it down using an shRNA adenovirus or siRNA duplex in
mouse and human islets and β-cells. Inhibition of PI3Kγ blunted the exocytotic and …
independent activity in β-cells and can be activated by the glucose-dependent insulinotropic
polypeptide (GIP). We therefore investigated the role of the PI3Kγ catalytic subunit (p110γ) in
insulin secretion and β-cell exocytosis stimulated by GIP. We inhibited p110γ with
AS604850 (1 µmol/liter) or knocked it down using an shRNA adenovirus or siRNA duplex in
mouse and human islets and β-cells. Inhibition of PI3Kγ blunted the exocytotic and …
