[HTML][HTML] Glucagon-like peptide 1 stimulates post-translational activation of glucokinase in pancreatic β cells
SY Ding, A Nkobena, CA Kraft, ML Markwardt… - Journal of Biological …, 2011 - ASBMB
Glucagon-like peptide 1 (GLP-1) potentiates glucose-stimulated insulin secretion from
pancreatic β cells, yet does not directly stimulate secretion. The mechanisms underlying this
phenomenon are incompletely understood. Here, we report that GLP-1 augments glucose-
dependent rises in NAD (P) H autofluorescence in both βTC3 insulinoma cells and islets in a
manner consistent with post-translational activation of glucokinase (GCK). GLP-1 treatment
increased GCK activity and enhanced GCK S-nitrosylation in βTC3 cells. A 2-fold increase in …
pancreatic β cells, yet does not directly stimulate secretion. The mechanisms underlying this
phenomenon are incompletely understood. Here, we report that GLP-1 augments glucose-
dependent rises in NAD (P) H autofluorescence in both βTC3 insulinoma cells and islets in a
manner consistent with post-translational activation of glucokinase (GCK). GLP-1 treatment
increased GCK activity and enhanced GCK S-nitrosylation in βTC3 cells. A 2-fold increase in …
