[HTML][HTML] COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes
K Matsuoka, L Orci, M Amherdt, SY Bednarek… - Cell, 1998 - cell.com
K Matsuoka, L Orci, M Amherdt, SY Bednarek, S Hamamoto, R Schekman, T Yeung
Cell, 1998•cell.comCOPII vesicle formation requires only three coat assembly subunits: Sar1p, Sec13/31p, and
Sec23/24p. PI 4-phosphate or PI 4, 5-bisphosphate is required for the binding of these
proteins to liposomes. The GTP-bound form of Sar1p recruits Sec23/24p to the liposomes as
well as to the ER membranes, and this Sar1p-Sec23/24p complex is required for the binding
of Sec13/31p. Ultrastructural analysis shows that the binding of COPII coat proteins to
liposomes results in coated patches, coated buds, and coated vesicles of 50–90 nm in …
Sec23/24p. PI 4-phosphate or PI 4, 5-bisphosphate is required for the binding of these
proteins to liposomes. The GTP-bound form of Sar1p recruits Sec23/24p to the liposomes as
well as to the ER membranes, and this Sar1p-Sec23/24p complex is required for the binding
of Sec13/31p. Ultrastructural analysis shows that the binding of COPII coat proteins to
liposomes results in coated patches, coated buds, and coated vesicles of 50–90 nm in …
Abstract
COPII vesicle formation requires only three coat assembly subunits: Sar1p, Sec13/31p, and Sec23/24p. PI 4-phosphate or PI 4,5-bisphosphate is required for the binding of these proteins to liposomes. The GTP-bound form of Sar1p recruits Sec23/24p to the liposomes as well as to the ER membranes, and this Sar1p-Sec23/24p complex is required for the binding of Sec13/31p. Ultrastructural analysis shows that the binding of COPII coat proteins to liposomes results in coated patches, coated buds, and coated vesicles of 50–90 nm in diameter. Budding proceeds without rupture of the donor liposome or vesicle product. These observations suggest that the assembly of the COPII coat on the ER occurs by a sequential binding of coat proteins to specific lipids and that this assembly promotes the budding of COPII-coated vesicles.
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